ngf stimulated pc12 cells (Santa Cruz Biotechnology)
Santa Cruz Biotechnology is a verified supplier 
90
Structured Review
Santa Cruz Biotechnology
ngf stimulated pc12 cells
Ngf Stimulated Pc12 Cells, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ngf+stimulated+pc12+cells/PC-12+%2B+NGF+Cell+Lysate/pm10224075-77-5-23
Average 90 stars, based on 1 article reviews
Ngf Stimulated Pc12 Cells, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ngf+stimulated+pc12+cells/PC-12+%2B+NGF+Cell+Lysate/pm10224075-77-5-23
Average 90 stars, based on 1 article reviews
ngf stimulated pc12 cells - by Bioz Stars,
2026-09
90/100 stars
Images
1) Product Images from "Nerve growth factor-stimulated B-Raf catalytic activity is refractory to inhibition by cAMP-dependent protein kinase."
Article Title: Nerve growth factor-stimulated B-Raf catalytic activity is refractory to inhibition by cAMP-dependent protein kinase.
Journal: The Journal of biological chemistry
doi: 10.1074/jbc.274.19.13193
Figure Legend Snippet: FIG. 3. The catalytic activity of B-Rafcat is inhibited by co- transfection of PKAcat in PC12 cells. A, a plasmid encoding B- Rafcat-GST was expressed in PC12 cells in the absence (2PKA) or presence (1PKA) of co-transfected PKAcat. MEK kinase activity of the glutathione-Sepharose-purified proteins was determined as described in Fig. 2. An anti-B-Raf immunoblot analysis of the samples used for the MEK kinase assay indicates that an equal amount of B-Rafcat-GST fusion protein was present in each reaction. B, a histogram summariz- ing the inhibitory effect of PKAcat on B-Rafcat activity in transfected PC12 cells from four independent experiments. The error bar indicates the standard deviation of the mean.
Techniques Used: Activity Assay, Cotransfection, Plasmid Preparation, Transfection, Purification, Western Blot, Kinase Assay, Standard Deviation
Figure Legend Snippet: FIG. 4. Activated B-Raf is not inhibited by PKA in vitro. A, activated B-Raf was immunoprecipitated from NGF-stimulated PC12 cells and incubated in the absence (2PKA) or presence (1PKA) of PKA in vitro. Raf-1 was isolated from immature Xenopus oocytes, activated with purified PKC, and subsequently treated in the presence or absence of PKA. MEK kinase activity of the B-Raf and Raf-1 proteins was measured as described in the legend to Fig. 2. In this experiment, B-Raf was activated by NGF to levels 4-fold higher than the activity of B-Raf in unstimulated PC12 cells. Raf-1 was activated by PKC 12.9-fold higher than the level of Raf-1 in immature Xenopus oocytes. KN-MEK, kinase-negative MEK. B, histograms summarizing the inhibitory effect of PKA on NGF-stimulated B-Raf activity and PKC-stimulated Raf-1 activity from three independent experiments. The error bars indicate the standard errors of the mean.
Techniques Used: In Vitro, Immunoprecipitation, Incubation, Isolation, Purification, Activity Assay
Figure Legend Snippet: FIG. 5. Full-length B-Raf is activated by co-expressed PKAcat in PC12 cells. Full-length B-Raf-GST was expressed in PC12 cells in the absence (2PKA) or presence (1PKA) of co-transfected PKAcat. A, B-Raf immunoblot analysis of glutathione-Sepharose affinity-purified lysates performed in the absence of bacterially expressed kinase-nega- tive MEK. B, MEK kinase activity of glutathione-Sepharose-purified proteins was measured as described in the legend to Fig. 2. The histo- gram summarizes the stimulatory effect of PKAcat on B-Raf-GST from three independent experiments. The error bar indicates the standard error of the mean.
Techniques Used: Transfection, Western Blot, Affinity Purification, Activity Assay, Purification
Related Articles
Activity Assay:Article Title: Nerve growth factor-stimulated B-Raf catalytic activity is refractory to inhibition by cAMP-dependent protein kinase. Article Snippet: After rocking at 4 °C for 1 h, the samples were spun at 6,000 3 g for 5 s. The pellets were washed twice in lysis buffer and once in Tris-buffered saline (20 mM Tris, pH7.5, 150 mM NaCl).After rocking at 4 °C for 1 h, the samples were spun at 6,000 3 g for 5 s. The pellets were washed twice in lysis buffer and once in Tris-buffered saline (20 mM Tris, pH7.5, 150 mM NaCl).. PC12 B-Raf was immunopurified from Cotransfection:Article Title: Nerve growth factor-stimulated B-Raf catalytic activity is refractory to inhibition by cAMP-dependent protein kinase. Article Snippet: After rocking at 4 °C for 1 h, the samples were spun at 6,000 3 g for 5 s. The pellets were washed twice in lysis buffer and once in Tris-buffered saline (20 mM Tris, pH7.5, 150 mM NaCl).After rocking at 4 °C for 1 h, the samples were spun at 6,000 3 g for 5 s. The pellets were washed twice in lysis buffer and once in Tris-buffered saline (20 mM Tris, pH7.5, 150 mM NaCl).. PC12 B-Raf was immunopurified from Plasmid Preparation:Article Title: Nerve growth factor-stimulated B-Raf catalytic activity is refractory to inhibition by cAMP-dependent protein kinase. Article Snippet: After rocking at 4 °C for 1 h, the samples were spun at 6,000 3 g for 5 s. The pellets were washed twice in lysis buffer and once in Tris-buffered saline (20 mM Tris, pH7.5, 150 mM NaCl).After rocking at 4 °C for 1 h, the samples were spun at 6,000 3 g for 5 s. The pellets were washed twice in lysis buffer and once in Tris-buffered saline (20 mM Tris, pH7.5, 150 mM NaCl).. PC12 B-Raf was immunopurified from Transfection:Article Title: Nerve growth factor-stimulated B-Raf catalytic activity is refractory to inhibition by cAMP-dependent protein kinase. Article Snippet: After rocking at 4 °C for 1 h, the samples were spun at 6,000 3 g for 5 s. The pellets were washed twice in lysis buffer and once in Tris-buffered saline (20 mM Tris, pH7.5, 150 mM NaCl).After rocking at 4 °C for 1 h, the samples were spun at 6,000 3 g for 5 s. The pellets were washed twice in lysis buffer and once in Tris-buffered saline (20 mM Tris, pH7.5, 150 mM NaCl).. PC12 B-Raf was immunopurified from Purification:Article Title: Nerve growth factor-stimulated B-Raf catalytic activity is refractory to inhibition by cAMP-dependent protein kinase. Article Snippet: After rocking at 4 °C for 1 h, the samples were spun at 6,000 3 g for 5 s. The pellets were washed twice in lysis buffer and once in Tris-buffered saline (20 mM Tris, pH7.5, 150 mM NaCl).After rocking at 4 °C for 1 h, the samples were spun at 6,000 3 g for 5 s. The pellets were washed twice in lysis buffer and once in Tris-buffered saline (20 mM Tris, pH7.5, 150 mM NaCl).. PC12 B-Raf was immunopurified from Western Blot:Article Title: Nerve growth factor-stimulated B-Raf catalytic activity is refractory to inhibition by cAMP-dependent protein kinase. Article Snippet: After rocking at 4 °C for 1 h, the samples were spun at 6,000 3 g for 5 s. The pellets were washed twice in lysis buffer and once in Tris-buffered saline (20 mM Tris, pH7.5, 150 mM NaCl).After rocking at 4 °C for 1 h, the samples were spun at 6,000 3 g for 5 s. The pellets were washed twice in lysis buffer and once in Tris-buffered saline (20 mM Tris, pH7.5, 150 mM NaCl).. PC12 B-Raf was immunopurified from Kinase Assay:Article Title: Nerve growth factor-stimulated B-Raf catalytic activity is refractory to inhibition by cAMP-dependent protein kinase. Article Snippet: After rocking at 4 °C for 1 h, the samples were spun at 6,000 3 g for 5 s. The pellets were washed twice in lysis buffer and once in Tris-buffered saline (20 mM Tris, pH7.5, 150 mM NaCl).After rocking at 4 °C for 1 h, the samples were spun at 6,000 3 g for 5 s. The pellets were washed twice in lysis buffer and once in Tris-buffered saline (20 mM Tris, pH7.5, 150 mM NaCl).. PC12 B-Raf was immunopurified from Standard Deviation:Article Title: Nerve growth factor-stimulated B-Raf catalytic activity is refractory to inhibition by cAMP-dependent protein kinase. Article Snippet: After rocking at 4 °C for 1 h, the samples were spun at 6,000 3 g for 5 s. The pellets were washed twice in lysis buffer and once in Tris-buffered saline (20 mM Tris, pH7.5, 150 mM NaCl).After rocking at 4 °C for 1 h, the samples were spun at 6,000 3 g for 5 s. The pellets were washed twice in lysis buffer and once in Tris-buffered saline (20 mM Tris, pH7.5, 150 mM NaCl).. PC12 B-Raf was immunopurified from In Vitro:Article Title: Nerve growth factor-stimulated B-Raf catalytic activity is refractory to inhibition by cAMP-dependent protein kinase. Article Snippet: After rocking at 4 °C for 1 h, the samples were spun at 6,000 3 g for 5 s. The pellets were washed twice in lysis buffer and once in Tris-buffered saline (20 mM Tris, pH7.5, 150 mM NaCl).After rocking at 4 °C for 1 h, the samples were spun at 6,000 3 g for 5 s. The pellets were washed twice in lysis buffer and once in Tris-buffered saline (20 mM Tris, pH7.5, 150 mM NaCl).. PC12 B-Raf was immunopurified from Immunoprecipitation:Article Title: Nerve growth factor-stimulated B-Raf catalytic activity is refractory to inhibition by cAMP-dependent protein kinase. Article Snippet: After rocking at 4 °C for 1 h, the samples were spun at 6,000 3 g for 5 s. The pellets were washed twice in lysis buffer and once in Tris-buffered saline (20 mM Tris, pH7.5, 150 mM NaCl).After rocking at 4 °C for 1 h, the samples were spun at 6,000 3 g for 5 s. The pellets were washed twice in lysis buffer and once in Tris-buffered saline (20 mM Tris, pH7.5, 150 mM NaCl).. PC12 B-Raf was immunopurified from Incubation:Article Title: Nerve growth factor-stimulated B-Raf catalytic activity is refractory to inhibition by cAMP-dependent protein kinase. Article Snippet: After rocking at 4 °C for 1 h, the samples were spun at 6,000 3 g for 5 s. The pellets were washed twice in lysis buffer and once in Tris-buffered saline (20 mM Tris, pH7.5, 150 mM NaCl).After rocking at 4 °C for 1 h, the samples were spun at 6,000 3 g for 5 s. The pellets were washed twice in lysis buffer and once in Tris-buffered saline (20 mM Tris, pH7.5, 150 mM NaCl).. PC12 B-Raf was immunopurified from Isolation:Article Title: Nerve growth factor-stimulated B-Raf catalytic activity is refractory to inhibition by cAMP-dependent protein kinase. Article Snippet: After rocking at 4 °C for 1 h, the samples were spun at 6,000 3 g for 5 s. The pellets were washed twice in lysis buffer and once in Tris-buffered saline (20 mM Tris, pH7.5, 150 mM NaCl).After rocking at 4 °C for 1 h, the samples were spun at 6,000 3 g for 5 s. The pellets were washed twice in lysis buffer and once in Tris-buffered saline (20 mM Tris, pH7.5, 150 mM NaCl).. PC12 B-Raf was immunopurified from Affinity Purification:Article Title: Nerve growth factor-stimulated B-Raf catalytic activity is refractory to inhibition by cAMP-dependent protein kinase. Article Snippet: After rocking at 4 °C for 1 h, the samples were spun at 6,000 3 g for 5 s. The pellets were washed twice in lysis buffer and once in Tris-buffered saline (20 mM Tris, pH7.5, 150 mM NaCl).After rocking at 4 °C for 1 h, the samples were spun at 6,000 3 g for 5 s. The pellets were washed twice in lysis buffer and once in Tris-buffered saline (20 mM Tris, pH7.5, 150 mM NaCl).. PC12 B-Raf was immunopurified from |